28S ribosomal RNA
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Through Phylogenetic tree, Protist and Fungus
Combined with 5.8S rRNA to the 5' side, it is the eukaryotic nuclear homologue of the prokaryotic 23S and mitochondrial 16S ribosomal RNAs.
Use in phylogeny
The genes coding for 28S rRNA are referred to as 28S rDNA. The comparison of the sequences from these genes are sometimes used in molecular analysis to construct phylogenetic trees, for example in protists, fungi, insects, arachnids, tardigrades, and vertebrates.
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Structure
The 28S rRNA is typically 4000–5000 nt long.
Some eukaryotes cleave 28S rRNA into two parts before assembling both into the ribosome, a phenomenon termed the "hidden break".
Databases
Several databases provide alignments and annotations of LSU rRNA sequences for comparative purposes:
- RDP, the Ribosomal Database Project;
- SILVA, a ribosomal RNA gene database project.