Ganglionic eminence

The eminence is divided into three regions of the ventral ventricular zone of the telencephalon (a lateral, medial and caudal eminence), where they facilitate tangential cell migration during embryonic development. Tangential migration does not involve interactions with radial glial cells; instead the interneurons migrate perpendicularly through the radial glial cells to reach their final location. The characteristics and function of the cells that follow the tangential migration pathway seem to be closely related to the location and precise timing of their production, and the GEs contribute significantly to building up the GABAergic cortical cell population. Another structure that the GEs contribute to is the basal ganglia. The GEs also guide the axons growing from the thalamus into the cortex and vice versa.

In humans, the GEs disappear by one year of age. During development, neuronal migration continues until the extinction of the germ layer, at which point the remnants from the germ layer make up the eminences.

Categorization

Ganglionic eminences are categorized into three groups based on their location within the subventricular zone:

• Medial ganglionic eminence (MGE)
• Lateral ganglionic eminence (LGE)
• Caudal ganglionic eminence (CGE)

A sulcus separates the medial and lateral ganglionic eminences. The expression of Nkx2-1, Gsx2, and Pax6 is required to determine the independent progenitor cell populations in the LGE and MGE. Interactions between these three genes define the boundaries between the different progenitor zones and mutations of these genes can cause abnormal expansion around the MGE, LGE, ventral pallium (VP), and anterior entopeduncular region (AEP). The cells of the GEs are quite homogenous, with the MGE, LGE, and CGE all having small, dark, irregular nuclei and moderately dense cytoplasm, however, each eminence can be identified by the type of progeny that it produces. See the individual GE sections below for more information on the different types of progeny produced.

Additionally, the subventricular zone is the starting point of multiple streams of tangentially migrating interneurons that express Dlx genes. There are three main tangential migration pathways that have been identified in this region:

1. the latero-caudal migration (subpallial telencephalon to cortex)
2. the medio-rostral migration (subpallial basal telencephalon to the olfactory bulb)
3. the latero-caudal migration (basal telencephalon to the striatum)

These pathways are temporally and spatially distinct, and produce a variety of GABAergic, and non-GABAergic interneurons. One example of GABAergic interneurons that the GEs guide are parvalbumin-containing interneurons in the neocortex. Some examples of non-GABAergic interneurons that the GEs guide are dopaminergic interneurons in the olfactory bulb, and cholinergic interneurons in the striatum. Cells migrating along these pathways move at different rates. Some molecules that have been implicated in controlling the rate of the unidirectional movement of cells derived from the GEs are hepatocyte growth factor/scattered factor (HGF/SF), and various neurotrophic factors.

Medial ganglionic eminence (MGE)

The primary purpose of the MGE during development is to produce GABAergic stellate cells and direct their migration to the neocortex. The precursors of most GABAergic interneurons in the cerebral cortex migrate from the subcortical progenitor zone. More specifically, performing a mechanical transection of the migratory route from the MGE to the neocortex causes a 33% decrease in GABAergic interneurons in the neocortex. The MGE also produces some of the neurons and glia of the basal ganglia and hippocampus. The MGE may also be a source of Cajal-Retzius cells, but this remains controversial. Early in embryonic development, the interneurons in the cortex stem primarily from the MGE and the AEP. In vitro experiments show that MGE cells migrate more than 300 μm per day, three times faster than the migration of LGE cells. See more about the time frame and function of MGE in comparison to the LGE in the following section.

Lateral ganglionic eminence (LGE)

Compared to the early temporal frame of development in the MGE, the LGE aids in the tangential migration of cells later in the mid-embryogenic stage. Unlike the MGE, which guides most cell migration into the cortex during this stage, the LGE contributes less to cell migration to the cortex, and instead guides many cells to the olfactory bulbs. In fact, the migration to the olfactory bulb is led by the LGE into adulthood. The route that newly generated neurons take from the anterior subventricular zone to the olfactory bulb is called the rostral migratory stream. During the late stages of embryonic development, both the LGE and MGE guide cell migration to the cortex, specifically the proliferative regions of the cortex. Some studies have found that the LGE also contributes cells to the neocortex, but this remains an issue of debate. In vitro, cells migrating from the LGE travel at a rate of 100 μm per day, slower than the MGE cells.

Caudal ganglionic eminence (CGE)

The caudal ganglionic eminence is another subcortical structure that is essential to the generation of cortical interneurons. It is located next to the lateral ventricle, posterior to where the LGE and MGE fuse. The CGE is a fusion of the rostral medial and lateral ganglionic eminence, which begins at the mid to caudal thalamus. There are two molecular domains that exist within the CGE and closely resemble extensions of the caudal MGE and LGE. The CGE is distinct from the LGE and MGE in gene expression patterns and progeny produced. Unlike the cells from the MGE, the cells from the CGE were rarely parvalbumin-containing neurons. It seems that the majority of cells from the CGE were GABAergic interneurons, but depending on where they are located, CGE-derived cells are very diverse. CGE-derived cells include GABAergic interneurons, spiny interneurons, mossy cells, pyramidal and granule neurons, and even oligodendrocyte and astrocyte glial cells.