Gene Ontology

Terms and ontology

From a practical view, an ontology is a representation of something we know about. "Ontologies" consist of representations of things that are detectable or directly observable and the relationships between those things. There is no universal standard terminology in biology and related domains, and term usage may be specific to a species, research area, or even a particular research group. This makes communication and sharing of data more difficult. The Gene Ontology project provides an ontology of defined terms representing gene product properties. The ontology covers three domains:

• cellular component, the parts of a cell or its extracellular environment;
• molecular function, the elemental activities of a gene product at the molecular level, such as binding or catalysis;
• biological process, operations or sets of molecular events with a defined beginning and end, pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms.

Each GO term within the ontology has a term name, which may be a word or string of words; a unique alphanumeric identifier; a definition with cited sources; and an ontology indicating the domain to which it belongs. Terms may also have synonyms, which are classed as being exactly equivalent to the term name, broader, narrower, or related; references to equivalent concepts in other databases; and comments on term meaning or usage. The GO is structured as a directed acyclic graph, and each term has defined relationships to one or more other terms in the same domain, and sometimes to other domains. The GO vocabulary is designed to be species-neutral and includes terms applicable to prokaryotes and eukaryotes, single and multicellular organisms.

GO is not static, and additions, corrections, and alterations are suggested by and solicited from members of the research and annotation communities, as well as by those directly involved in the GO project. For example, an annotator may request a specific term to represent a metabolic pathway, or a section of the ontology may be revised with the help of community experts (e.g.). Suggested edits are reviewed by the ontology editors, and implemented where appropriate.

The GO and annotation files are freely available from the GO website in a number of formats or can be accessed online using the GO browser AmiGO. The Gene Ontology project also provides downloadable mappings of its terms to other classification systems.

Example term

id: GO:0000016

name: lactase activity

ontology: molecular_function

def: "Catalysis of the reaction: lactose + H2O=D-glucose + D-galactose." [EC:3.2.1.108]

synonym: "lactase-phlorizin hydrolase activity" BROAD [EC:3.2.1.108]

synonym: "lactose galactohydrolase activity" EXACT [EC:3.2.1.108]

xref: EC:3.2.1.108

xref: MetaCyc:LACTASE-RXN

xref: Reactome:20536

is_a: GO:0004553 ! hydrolase activity, hydrolyzing O-glycosyl compounds

Data source:

Annotation

Genome annotation encompasses the practice of capturing data about a gene product, and GO annotations use terms from the GO to do so. Annotations from GO curators are integrated and disseminated on the GO website, where they can be downloaded directly or viewed online using AmiGO. In addition to the gene product identifier and the relevant GO term, GO annotations have at least the following data: The reference used to make the annotation (e.g. a journal article); An evidence code denoting the type of evidence upon which the annotation is based; The date and the creator of the annotation

Supporting information, depending on the GO term and evidence used, and supplementary information, such as the conditions the function is observed under, may also be included in a GO annotation.

The evidence code comes from a controlled vocabulary of codes, the Evidence Code Ontology, covering both manual and automated annotation methods. For example, Traceable Author Statement (TAS) means a curator has read a published scientific paper and the metadata for that annotation bears a citation to that paper; Inferred from Sequence Similarity (ISS) means a human curator has reviewed the output from a sequence similarity search and verified that it is biologically meaningful. Annotations from automated processes (for example, remapping annotations created using another annotation vocabulary) are given the code Inferred from Electronic Annotation (IEA). In 2010, over 98% of all GO annotations were inferred computationally, not by curators, but as of July 2, 2019, only about 30% of all GO annotations were inferred computationally. As these annotations are not checked by a human, the GO Consortium considers them to be marginally less reliable and they are commonly to a higher level, less detailed terms. Full annotation data sets can be downloaded from the GO website. To support the development of annotation, the GO Consortium provides workshops and mentors new groups of curators and developers.

Many machine learning algorithms have been designed and implemented to predict Gene Ontology annotations.

Example annotation

Gene product: Actin, alpha cardiac muscle 1, UniProtKB:P68032

GO term: heart contraction; GO:0060047 (biological process)

Evidence code: Inferred from Mutant Phenotype (IMP)

Reference: PMID 17611253

Assigned by: UniProtKB, June 6, 2008

Data source:

Tools

There are a large number of tools available, both online and for download, that use the data provided by the GO project. The vast majority of these come from third parties; the GO Consortium develops and supports two tools, AmiGO and OBO-Edit.

AmiGO is a web-based application that allows users to query, browse, and visualize ontologies and gene product annotation data. It also has a BLAST tool, tools allowing analysis of larger data sets, and an interface to query the GO database directly. AmiGO can be used online at the GO website to access the data provided by the GO Consortium or downloaded and installed for local use on any database employing the GO database schema (e.g.). It is free open source software and is available as part of the go-dev software distribution.

OBO-Edit is an open source, platform-independent ontology editor developed and maintained by the Gene Ontology Consortium. It is implemented in Java and uses a graph-oriented approach to display and edit ontologies. OBO-Edit includes a comprehensive search and filter interface, with the option to render subsets of terms to make them visually distinct; the user interface can also be customized according to user preferences. OBO-Edit also has a reasoner that can infer links that have not been explicitly stated based on existing relationships and their properties. Although it was developed for biomedical ontologies, OBO-Edit can be used to view, search, and edit any ontology. It is freely available to download.

Consortium

The Gene Ontology Consortium is the set of biological databases and research groups actively involved in the gene ontology project. This includes a number of model organism databases and multi-species protein databases, software development groups, and a dedicated editorial office.