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Genetically modified brinjal

Variety of brinjal

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Through Transgene, Bacteria and Bacillus thuringiensis

The genetically modified brinjal event is termed Event EE 1, and Mahyco has also applied for approval of two brinjal hybrids. Event EE 1 was introduced by breeding the plants into various local varieties by University of Agricultural Sciences, Dharwad and Tamil Nadu Agricultural University, Coimbatore. Some of the cultivars of Brinjal include Malpur local, Manjari gota, Kudachi local, Udupi local, 112 GO, and Pabkavi local. It was approved for commercialization in India in 2009, but after a public outcry and rounds of debates in which representatives from Mahyco, the scientific community, and non-governmental organizations spoke on the topic, the Indian Environment Minister, Jairam Ramesh, facilitated a moratorium on its release until further unspecified tests were conducted. Bt brinjal was approved for commercial release in Bangladesh in 2013.

By 2021, it had been cultivated by nearly 65,000 farmers with an approximate 6x net return, and about 20% of farmers used seeds from previous seasons.

Development

Mahyco licensed and used the cry1Ac gene obtained from Monsanto and two supporting genes (nptII and aad). The cry1Ac gene is under the transcriptional control of an enhanced cauliflower mosaic virus 35S (CaMV35S) promoter, which ensures the gene is expressed in all the brinjal's tissue throughout its complete life cycle. NptII and aad are selectable marker genes, nptII is used to identify transgenic plants from non-transgenic, and aad is used to identify the transformed bacteria used during the development of the construct. Aad contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an Agrobacterium-mediated technique. Agrobacterium naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion.

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