Homoserine
- 1 min read
- 2 chapters
Related on Lably
Through Amino acid, Chemical formula and Peptide
Homoserine is an intermediate in the biosynthesis of three essential amino acids: methionine, threonine (an isomer of homoserine), and isoleucine. Its complete biosynthetic pathway includes glycolysis, the tricarboxylic acid (TCA) or citric acid cycle (Krebs cycle), and the aspartate metabolic pathway. It forms by two reductions of aspartic acid via the intermediacy of aspartate semialdehyde. Specifically, the enzyme homoserine dehydrogenase, in association with NADPH, catalyzes a reversible reaction that interconverts L-aspartate-4-semialdehyde to L-homoserine. Then, two other enzymes, homoserine kinase and homoserine O-succinyltransferase use homoserine as a substrate and produce phosphohomoserine and O-succinyl homoserine respectively.
Applications
Commercially, homoserine can serve as precursor to the synthesis of isobutanol and 1,4-butanediol. Purified homoserine is used in enzyme structural studies. Also, homoserine has played important roles in studies to elucidate peptide synthesis and synthesis of proteoglycan glycopeptides. Bacterial cell lines can make copious amounts of this amino acid.
Sign in to keep reading
One more chapter is free with a Lably account.
Biosynthesis
Homoserine is produced from aspartate via aspartate-4-semialdehyde, which is produced from β-phosphoaspartate. By the action of homoserine dehydrogenases, the semialdehyde is converted to homoserine.
L-Homoserine is substrate for homoserine kinase, yielding phosphohomoserine (homoserine-phosphate), which is converted to by threonine synthase to yield L-threonine.
Homoserine is converted to O-succinyl homoserine by homoserine O-succinyltransferase, a precursor to L-methionine.
Homoserine allosterically inhibits aspartate kinase and glutamate dehydrogenase. Glutamate dehydrogenase reversibly converts glutamate to α-ketoglutarate and α-ketoglutarate coverts to oxaloacetate through the citric cycle. Threonine acts as another allosteric inhibitor of aspartate kinase and homoserine dehydrogenase, but it is a competitive inhibitor of homoserine kinase.