Overlapping gene

Evolution

Overlapping genes are particularly common in rapidly evolving genomes, such as those of viruses, bacteria, and mitochondria. They may originate in three ways:

1. By extension of an existing open reading frame (ORF) downstream into a contiguous gene due to the loss of a stop codon;
2. By extension of an existing ORF upstream into a contiguous gene due to loss of an initiation codon;
3. By generation of a novel ORF within an existing one due to a point mutation.

The use of the same nucleotide sequence to encode multiple genes may provide evolutionary advantage due to reduction in genome size and due to the opportunity for transcriptional and translational co-regulation of the overlapping genes. Gene overlaps introduce novel evolutionary constraints on the sequences of the overlap regions.

Origins of new genes

In 1977, Pierre-Paul Grassé proposed that one of the genes in the pair could have originated de novo by mutations to introduce novel ORFs in alternate reading frames; he described the mechanism as overprinting. It was later substantiated by Susumu Ohno, who identified a candidate gene that may have arisen by this mechanism. Some de novo genes originating in this way may not remain overlapping, but subfunctionalize following gene duplication, contributing to the prevalence of orphan genes. Which member of an overlapping gene pair is younger can be identified bioinformatically either by a more restricted phylogenetic distribution, or by less optimized codon usage. Younger members of the pair tend to have higher intrinsic structural disorder than older members, but the older members are also more disordered than other proteins, presumably as a way of alleviating the increased evolutionary constraints posed by overlap. Overlaps are more likely to originate in proteins that already have high disorder.

Taxonomic distribution

Overlapping genes occur in all domains of life, though with varying frequencies. They are especially common in viral genomes.

Viruses

The existence of overlapping genes was first identified in the virus ΦX174, whose genome was the first DNA genome ever sequenced by Frederick Sanger in 1977. Previous analysis of ΦX174, a small single-stranded DNA bacteriophage that infected the bacteria Escherichia coli, suggested that the proteins produced during infection required coding sequences longer than the measured length of its genome. Analysis of the fully sequenced 5386 nucleotide genome showed that the virus possessed extensive overlap between coding regions, revealing that some genes (like genes D and E) were translated from the same DNA sequences but in different reading frames. An alternative start site within the genome replication gene A of ΦX174 was shown to express a truncated protein with an identical coding sequence to the C-terminus of the original A protein but possessing a different function It was concluded that other undiscovered sites of polypeptide synthesis could be hidden through the genome due to overlapping genes. An identified de novo gene of another overlapping gene locus was shown to express a novel protein that induces lysis of E. coli by inhibiting biosynthesis of its cell wall[56], suggesting that de novo protein creation through the process of overprinting can be a significant factor in the evolution of pathogenicity of viruses. Another example is the ORF3d gene in the SARS-CoV 2 virus. Overlapping genes are particularly common in viral genomes. Some studies attribute this observation to selective pressure toward small genome sizes mediated by the physical constraints of packaging the genome in a viral capsid, particularly one of icosahedral geometry. However, other studies dispute this conclusion and argue that the distribution of overlaps in viral genomes is more likely to reflect overprinting as the evolutionary origin of overlapping viral genes. Overprinting is a common source of de novo genes in viruses.

The proportion of viruses with overlapping coding sequences within their genomes varies. Double-stranded RNA viruses have fewer than a quarter that contains them while almost three-quarters of retroviridae and viruses with single-stranded DNA genomes contain overlapping coding sequences. Segmented viruses in particular, or viruses with their genome split into separate pieces and packaged either all in the same capsid or in separate capsids, are more likely to contain an overlapping sequence than non-segmented viruses. RNA viruses have fewer overlapping genes than DNA viruses which possess lower mutation rates and less restrictive genome sizes. The lower mutation rate of DNA viruses facilitates greater genomic novelty and evolutionary exploration within a structurally constrained genome and may be the primary driver of the evolution of overlapping genes.

Studies of overprinted viral genes suggest that their protein products tend to be accessory proteins which are not essential to viral proliferation, but contribute to pathogenicity. Overprinted proteins often have unusual amino acid distributions and high levels of intrinsic disorder. In some cases overprinted proteins do have well-defined, but novel, three-dimensional structures; one example is the RNA silencing suppressor p19 found in Tombusviruses, which has both a novel protein fold and a novel binding mode in recognizing siRNAs.

Prokaryotes

Estimates of gene overlap in bacterial genomes typically find that around one third of bacterial genes are overlapped, though usually only by a few base pairs. Most studies of overlap in bacterial genomes find evidence that overlap serves a function in gene regulation, permitting the overlapped genes to be transcriptionally and translationally co-regulated. In prokaryotic genomes, unidirectional overlaps are most common, possibly due to the tendency of adjacent prokaryotic genes to share orientation. Among unidirectional overlaps, long overlaps are more commonly read with a one-nucleotide offset in reading frame (i.e., phase 1) and short overlaps are more commonly read in phase 2. Long overlaps of greater than 60 base pairs are more common for convergent genes; however, putative long overlaps have very high rates of misannotation. Robustly validated examples of long overlaps in bacterial genomes are rare; in the well-studied model organism Escherichia coli, only four gene pairs are well validated as having long, overprinted overlaps.

Eukaryotes

Compared to prokaryotic genomes, eukaryotic genomes are often poorly annotated and thus identifying genuine overlaps is relatively challenging. However, examples of validated gene overlaps have been documented in a variety of eukaryotic organisms, including mammals such as mice and humans. Eukaryotes differ from prokaryotes in distribution of overlap types: while unidirectional (i.e., same-strand) overlaps are most common in prokaryotes, opposite or antiparallel-strand overlaps are more common in eukaryotes. Among the opposite-strand overlaps, convergent orientation is most common. Most studies of eukaryotic gene overlap have found that overlapping genes are extensively subject to genomic reorganization even in closely related species, and thus the presence of an overlap is not always well-conserved. Overlap with older or less taxonomically restricted genes is also a common feature of genes likely to have originated de novo in a given eukaryotic lineage.

Function

The precise functions of overlapping genes seems to vary across the domains of life but several experiments have shown that they are important for virus lifecycles through proper protein expression and stoichiometry as well as playing a role in proper protein folding. A version of bacteriophage ΦX174 has also been created where all gene overlaps were removed proving they were not necessary for replication.

The retention and evolution of overlapping genes within viruses may also be due to capsid size limitations. Dramatic viability loss was observed in viruses with genomes engineered to be longer than the wild-type genome. Increasing the single-stranded DNA genome length of ΦX174 by >1% results in almost complete loss of infectivity, believed to be the result of the strict physical constraints imposed by the finite capsid volume. Studies on adeno-associated viruses as gene delivery vectors showed that viral packaging is constrained by genetic cargo size limits, requiring the use of multiple vectors to deliver large human genes such as CFTR81. Therefore, it is suggested that overlapping genes evolved as a means to overcome these physical constraints, increasing genetic diversity by utilizing only the existing sequence rather than increasing genome length.

Methods in identifying overlapping genes and ORFs

Standardized methods such as genome annotation may be inappropriate for the detection of overlapping genes as they are reliant on already curated genes while overlapping genes are generally overlooked contain atypical sequence composition. Genome annotation standards are also often biased against feature overlaps, such as genes entirely contained within another gene. Furthermore, some bioinformatics pipelines such as the RAST pipeline markedly penalizes overlaps between predicted ORFs. However, rapid advancement of genome-scale protein and RNA measurement tools along with increasingly advanced prediction algorithms have revealed an avalanche of overlapping genes and ORFs within numerous genomes. Proteogenomic methods have been essential in discovering numerous overlapping genes and include a combination of techniques such as bottom-up proteomics, ribosome profiling, DNA sequencing, and perturbation. RNA sequencing is also used to identify genomic regions containing overlapping transcripts. It has been utilized to identify 180,000 alternate ORFs within previously annotated coding regions found in humans. Newly discovered ORFs such as these are verified using a variety of reverse genetics techniques, such as CRISPR-Cas9 and catalytically dead Cas9 (dCas9) disruption. Attempts at proof-by-synthesis are also performed to show beyond doubt the absence of any undiscovered overlapping genes.