Q-system (genetics)
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Through Genetics, Gene expression and Transgene
Origin
The Q-system is based on two out of the seven genes of the qa gene cluster of the bread fungus Neurospora crassa. The genes of the qa cluster are responsible for the catabolism of quinic acid, which is used by the fungus as a carbon source in conditions of low glucose. The cluster contains a transcriptional activator qa-1F, a transcriptional repressor qa-1S, and five structural genes. The qa-1F binds to a specific DNA sequence, found upstream of the qa genes. The presence of quinic acid disrupts interaction between qa-1F and qa-1S, thus disinhibiting the transcriptional activity of qa-1F. Genes qa-1F, qa-1S and the DNA binding sequence of qa-1F form the basis of the Q-system. The genes were renamed to simplify their use as follows: transcriptional activator qa-1F as QF, repressor qa-1S as QS, and the DNA binding sequence as QUAS. The quinic acid represents the fourth component of the Q-system. The original transactivator QF appeared to be toxic when expressed broadly in Drosophila. To overcome this problem, two new transactivators were developed: QF2 and QF2w.
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Use in Drosophila
Basic use
The Q-system functions similarly to, and independently of, the GAL4/UAS and the LexA/LexAop systems. QF, QF2 and QF2w are analogous to GAL4 and LexA, and their expression is usually under the control of cell-type specific promoter, such as nsyb (to target neurons) or tubulin (to target all cells). QUAS is analogous to UAS and LexAop, and is placed upstream of an effector gene, such as GFP. QS is analogous to GAL80, and may be driven by any promoter (e.g. tubulin-QS). Quinic acid is a unique feature of the Q-system, and it must be fed to the flies or maggots in order to alleviate the QS-induced repression. In some ways, quinic acid is analogous to temperature in the case of GAL80ts. In its basic form, two transgenic fly lines, one containing a QF transgene and the other one containing a QUAS transgene, are crossed together. Their progeny that had both a QF transgene and a QUAS transgene will be expressing a reporter gene in a subset of cells (e.g. nsyb-QF2, QUAS-GFP flies express GFP in all neurons). If a fly also expresses QS in some of the cells, the activity of QF will be repressed in these cells, but it may be restored of a fly is fed quinic acid (e.g. a nsyb-QF2, QUAS-GFP, tub-QS fly expresses no GFP when its diet doesn't contain quinic acid, and expresses GFP in its neurons when fed quinic acid). The use of QS repressor and quinic acid allows to fine-tune the temporal control of transgene expression.
Chimeric transactivators
Chimeric transactivators GAL4QF and LexAQF allow to combine the use of all three binary expression systems. GAL4QF binds to UAS, and may be repressed by QS while being unaffected by GAL80. Similarly, LexAQF binds to LexAop, and may be repressed by QS. LexAQF represents a useful extension of the LexA/LexAop system that doesn't have its own repressor.
Intersectional expression
A variety of expression patterns may be achieved by combination of the three binary expression systems and the FLP/FRT or other recombinases. Expression patterns may be constructed as AND, OR, NOR etc. logic gates to e.g. narrow down expression patterns of available GAL4 lines. The resulting expression pattern somewhat depends on the developmental timing of activation of the transcription factors (discussed in ).
Use in other organisms
Q-system appeared to be working successfully in a variety of organisms. It has been used to drive expression of luciferase, as a proof of principle, in cultured mammalian cells. In zebrafish the Q-system has been successfully used with several tissue-specific promoters, and was shown to work independently of the GAL4/UAS system when expressed in the same cell. In C. elegans the Q-system has been shown to work in muscles and in neuronal tissue. In 2016, the Q-system was used to target, for the first time, the olfactory neurons of malaria mosquitoes Anopheles gambiae. In 2019, the Q-system in Anopheles mosquitoes was used to examine the functional responses of olfactory neurons to odors. In 2019, the Q-system was introduced into the Aedes aegypti mosquito to capture tissue specific expression patterns. These successes make the Q-system the system of choice when developing genetic tools for other organisms. Currently the main shortcoming of the Q-system is the low number of available transgenic lines, but it will be overcome as the scientific community creates and shares these resources, such as by the use of the GAL4>QF2 HACK system to convert existing GAL4 transgenic insertions to QF2. DNA binding domain of QF2 fused with VP16 transcriptional activator domain was successfully applied in Penicillium to gain control over the penicillin producing secondary metabolite gene cluster in a scalable manner.