Aplanochytrium

History of knowledge

The genus Aplanochytrium was first described in 1972 by Bahnweg. First included in the order thraustochytrids, Aplanochytrium is now considered a sister group of the thraustochytrids and Labyrinthula. The previous name of Aplanochytrium was Labyrinthuloides. Some species now considered part of Aplanochytrium were first classified in other groups, typically Labyrinthula and thraustochytrids and only reassigned later on into Aplanochytrium. An example is Aplanochytrium yorkensis, which was first considered as Labyrinthula and one year later reclassified into the genera Aplanochytrium.

Habitat and ecology

Aplanochytrium is exclusively marine and their isolates have been found in water samples, sediments, detritus, oyster mantle, gastropods and seagrasses. They can either be free-living in water or as symbiont in host organisms, where some species act as parasitic symbiont.

Description of the organism

Morphology and anatomy

The general shape of Aplanochytrium cells can be spherical to ovoid and their morphology is characterized by a single nucleus with a prominent nucleolus, numerous tubular mitochondria, a Golgi body and the presence of electron-dense regions at the periphery of the cell known as bothrosomes.

Cells belonging to the genus Aplanochytrium have an average length of 2.4-5 γm. The cell wall surrounding each cell is formed by multiple layers of circular scales mostly composed of fucose, similar to the one in Labyrinthula. They do use photosynthesis as energy source and thus they often have a white or cream colour.

A feature characteristic of Aplanochytrium is the production of the ectoplasmic net. The ectoplasmic net is an extension of the ectoplasm and limited by a plasma membrane and forming long threads. It is secreted and attached to the cell by bothrosomes, which are specialized organelles blocking the leaking of the organelles from the cell into the ectoplasmic net. The cells are not surrounded by the ectoplasmic net, unlike the Labyrinthula cells. The binding of the cells to the net allows a slow gliding movement of the cell along the threads.

Different types of morphology of the colonies have been seen. On one side, colonies producing broad rays of continuous band of cells and on the other side, patches of cells spreading from the edge of the colony outwards.

Organisms of different species do not move the same way. Some of them move slowly in a continuous movement, whereas other organisms like Aplanochytrium saliens move through intermittent quick advances. This locomotory style is slow, with a quick jump of 1-6 γm once a minute. Most of the cells leave slime trails behind them, which are passive marks of their passage, when they are gliding on the ectoplasmic net.

Life cycle

No sexual reproduction cells or structures have been identified yet, but the asexual reproduction cycle is well understood. Aplanochytrium multiply through spores produced by binary fission and held within the parent wall to form a spherical colorless sporangium. These daughter cells are called aplanospores and are not flagellated. Ten to fifty of these spores are released either due to the complete disintegration of the cell wall or through the production of tears at one or two points in the cell wall, through which the spores can leave the sporangium. The latter method of release enables the maintenance of an intact cell wall, which does not need to be synthesized de novo after each division. Once released, the aplanospores move away by gliding on their individual developed ectoplasmic net.

List of species