Phycobiliprotein
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Through Protein, Photosynthesis and Covalent bond
Major phycobiliproteins
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Characteristics
Phycobiliproteins demonstrate superior fluorescent properties compared to small organic fluorophores, especially when high sensitivity or multicolor detection required :
- Broad and high absorption of light suits many light sources
- Very intense emission of light: 10-20 times brighter than small organic fluorophores
- Relative large Stokes shift gives low background, and allows multicolor detections.
- Excitation and emission spectra do not overlap compared to conventional organic dyes.
- Can be used in tandem (simultaneous use by FRET) with conventional chromophores (i.e. PE and FITC, or APC and SR101 with the same light source).
- Longer fluorescence retention period.
- High water solubility
Applications
Phycobiliproteins allow very high detection sensitivity, and can be used in various fluorescence based techniques fluorimetric microplate assays Archived 2018-03-18 at the Wayback Machine, FISH and multicolor detection.
They are under development for use in artificial photosynthesis, limited by the relatively low conversion efficiency of 4-5%.
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