Nonsynaptic plasticity

Types

Intrinsic excitability of a neuron

The excitability of a neuron at any point depends on the internal and external conditions of the cell at the time of stimulation. Since a neuron typically receives multiple incoming signals at a time, the propagation of an action potential depends on the integration of all the incoming excitatory and inhibitory postsynaptic potentials arriving at the axon hillock. If the summation of all excitatory and inhibitory signals depolarize the cell membrane to the threshold voltage, an action potential is fired. Changing the intrinsic excitability of a neuron will change that neuron's function.

Spike generation

Nonsynaptic plasticity has an excitatory effect on the generation of spikes. The increase in spike generation has been correlated with a decrease in the spike threshold, a response from nonsynaptic plasticity. This response can result from the modulation of certain presynaptic K⁺ (potassium ion) currents (I_A, I_K,Ca, and I_Ks), which work to increase the excitability of the sensory neurons, broaden the action potential, and enhance neurotransmitter release. These modulations of K⁺ conductances serve as common mechanisms for regulating excitability and synaptic strength.

Regulation of synaptic plasticity

Nonsynaptic plasticity has been linked with synaptic plasticity, via both synergistic and regulatory mechanisms. The degree of synaptic modification determines the polarity of nonsynaptic changes, affecting the change in cellular excitability. Moderate levels of synaptic plasticity produce nonsynaptic changes that will synergistically act with the synaptic mechanisms to strengthen a response. Conversely, more robust levels of synaptic plasticity will produce nonsynaptic responses that will act as a negative feedback mechanism. The negative feedback mechanisms work to protect against saturation or suppression of the circuit activity as a whole.

Axonal modulation

Axonal modulation is a type of plasticity in which the number, activity, or location of ion channels in the axon changes. This causes the neuron to behave differently when stimulated. The modulation of ion channels is a response to a change in the stimulation frequencies of a neuron.

Propagation plasticity

Because it is the summation of the action potentials that eventually results in the threshold polarization being crossed, the temporal relationship of different input signals is very important in determining if and when a post-synaptic neuron will fire. Over time, the time it takes an action potential to propagate down the length of a particular axon can change. In one experiment multielectrode arrays were used to measure the time it took for action potentials to travel from one electrode to another, called latency. The neurons were then stimulated and the value of the latency was recorded over time. The latency values changed over time, suggesting that axonal plasticity influenced the propagation of action potentials.

Shunting

Shunting is a process in which axonal ion channels open during the passive flow (not requiring an ion pump) of a subthreshold depolarization down the axon. Usually occurring at axonal branch points, the timing of these channels opening as the subthreshold signal arrives in the area causes a hyperpolarization to be introduced to the passively flowing depolarization. Therefore, the cell is able to control which branches of the axon the subthreshold depolarization current flows through, resulting in some branches of the axon being more hyperpolarized than others. These differing membrane potentials cause certain areas of the neuron to be more excitable than others, based on the specific location and occurrence of shunting.

High frequency stimulation

Short-term effects: High frequency stimulation of a neuron for a short period of time increases the excitability of the neuron by lowering the amount of voltage required to fire an action potential. High frequency stimulation leads to an increase in the intracellular concentration of sodium and calcium ions due to the repeated opening of voltage-gated sodium and calcium channels in the axon and terminal. As the frequency of stimuli increases, there is less time between each stimulus for the cell to repolarize and return to normal resting potential. Therefore, the resting potential becomes more depolarized, meaning a smaller depolarizing current is needed to fire an action potential.

However, this modulation is usually very short lived. If the stimulation ceases, the neuron will revert to its original resting potential as the ion channels and pumps have ample time to recover from the last stimulus.

Long-term effects: High frequency stimulation of a neuron over a long period of time causes two resulting neuronal changes. Initially, the neuron responds as it would during short-term stimulation, with an increase in excitability. Continuing the high frequency stimulation after this point, results in a drastic, non-reversible change in excitability. When sodium concentrations reach a high enough level in the axon, sodium/calcium pumps reverse their direction of flow, causing calcium to be imported into the cell as sodium is exported out. The increased calcium concentration (and subsequent depolarization of the membrane) inactivates sodium channels and targets them for endocytosis and lysosomal hydrolysis. This results in a major decrease in axonal sodium channels, which are necessary for action potential propagation. If the stimulation continues, eventually the neuron will stop transmitting action potentials and will die. Neuronal death due to overstimulation is called excitotoxicity.

Low frequency stimulation

Short-term effects: All living neurons have a basal rate of action potential propagation and synaptic release. Thus, low frequency stimulation of a neuron in the short term is similar to the activity of a neuron at rest in the brain. No major changes happen to the intrinsic excitability of the neuron.

Long-term effects: Low frequency stimulation of a neuron for a long period of time decreases the excitability of the neuron by activating calcium-dependent phosphatases that tag AMPA receptors for internalization. Low frequency stimulation leads to low levels of calcium in the cell. When calcium concentrations are low, active calcium-dependent phosphatases dominate over calcium-dependent kinases. As more phosphatases are activated, they tag more AMPA receptors for internalization through endocytosis. Since AMPA receptors are one of the main excitatory receptors on neurons, removing them from the cell membrane effectively depresses the cell (if the cell cannot react to excitatory signals, it cannot generate an action potential of its own). In this way low frequency stimulation can actually reverse the effects of long-term potentiation, however these concepts are generally considered types of synaptic plasticity.

Homeostatic and Hebbian plasticity

Central nervous system (CNS) neurons integrate signals from many neurons. In the short term, it is important to have changes in activity of the neuron because this is how information is conveyed in the nervous system (Hebbian plasticity). However, for long-term sustainability, drift towards excitability or inexcitability will disturb the circuit's ability to convey information (homeostatic plasticity). Long-term potentiation (LTP) induces a higher firing rate in post synaptic neurons. It has been hypothesized that the intrinsic properties of a neuron should be arranged to make the most of the dynamic range, acting as a homeostatic mechanism. However, it was shown that intrinsic excitability follows a lognormal distribution which requires active, Hebbian learning to be kept up. In vitro studies have found that when the spontaneous activity of neuronal cultures is inhibited, the neurons become hyper excitable and that when an increase in activity is induced for long periods, the firing rates of the culture drop. In contrast, there is a wealth of evidence that the opposite form of regulation, Hebbian learning or LTP-IE/LTD-IE, also occurs and theoretical arguments show that Hebbian plasticity must be the dominant form of plasticity for intrinsic excitability as well. Since homeostatic plasticity also occurs between individual synapses, an earlier view suggesting that homeostatic plasticity and intrinsic plasticity are linked was shown to be inconsistent with evidence.

Mechanism

One mechanism for preserving the dynamic range of a neuron is synaptic scaling, a homeostatic form of plasticity that restores neuronal activity to its normal 'baseline' levels by changing the postsynaptic response of synapses of a neuron as a function of activity. Homeostatic modulation of the intrinsic excitability of a neuron is another way to maintain stability. The regulation of ionic conductances can be achieved in a number of ways, mostly through the release of neuromodulators like dopamine, serotonin etc. Another way is through the controlled release of brain-derived neurotrophic factor (BDNF). BDNF has also been found to influence synaptic scaling, suggesting that this neurotrophic factor may be responsible for the coordination of synaptic and nonsynaptic mechanisms in homeostatic plasticity.

Dendritic excitability

The dendrites are the regions responsible for the integration of the inputs from other neurons. One way that neurons manipulate the integration properties of the dendrites is by changing the number and properties of voltage gated ion channels. Inducing Long-term potentiation (LTP) in a particular synapse, results in an increase in excitability of the dendritic branches specific to that synapse. Dendritic excitability is important for the propagation and integration of synaptic signals. Dendritic excitability is thought to contribute to E-S potentiation, or an increase in the probability that a given input will result in the firing of an action potential.

It is known that changes in dendritic excitability affect action potential back propagation. Action potentials begin near the axon hillock and propagate down the length of the axon, but they also propagate backward through the soma into the dendritic arbor. Active back propagation is dependent on ion channels and changing the densities or properties of these channels can influence the degree to which the signal is attenuated. Plasticity of back-propagation in the dendrites occurs in less than one minute and lasts longer than 25 minutes. Back propagation is a method of signaling to the synapses that an action potential was fired. This is important for spike-timing-dependent plasticity. Fast dendritic adaptation on timescales of few seconds was experimentally observed indicating a potential meaningful global learning mechanism

Intrinsic plasticity

Intrinsic plasticity is a form of activity-dependent plasticity distinct from synaptic plasticity, which involves changes at the synapse between two neurons rather than changes in the electrical properties within a single neuron. There are some closely related phenomena that can affect a neuron's excitability – such as neuromodulation, structural plasticity, short-term plasticity due to channel kinetics, and neural development. There is no consensus on the quantity that intrinsic plasticity regulates, e.g. the firing rate of a neuron, its gain or its internal calcium concentration. Functionally, intrinsic plasticity might allow neurons to learn the intensity of stimuli and represent those intensity statistics in their excitabilities. Intrinsic plasticity contributes to encoding memory and complements other forms of activity-dependent plasticity including synaptic plasticity.

Higher brain function

Long-term associative memory

Experimental evidence

The experiment of Kemenes et al. demonstrated that in an extrinsic modulatory neuron, nonsynaptic plasticity influences the expression of long-term associative memory. The relationship between nonsynaptic plasticity and memory was assessed using cerebral giant cells (CGCs). Depolarization from conditioned stimuli increased the neuronal network response. This depolarization lasted as long as the long-term memory. Persistent depolarization and behavioral memory expression occurred more than 24 hours after training, indicating long-term effects. In this experiment, the electrophysiological expression of the long-term memory trace was a conditioned stimulus induced feeding response. CGCs were significantly more depolarized in the trained organisms than the control group, indicating association with learning and excitability changes. When CGCs were depolarized, they showed an increased response to the conditional stimuli and a stronger fictive feeding response. This demonstrated that the depolarization is enough to produce a significant feeding response to the conditioned stimuli. Additionally, no significant difference was observed in the feeding rates between conditioned organisms and ones that were artificially depolarized, reaffirming that depolarization is sufficient to generate the behavior associated with long-term memory.

Memory storage

Nonsynaptic activity in the cell is usually expressed as changes in neuronal excitability. This occurs through modulation of membrane components, such as resting and voltage-gated channels and ion pumps. Nonsynaptic processes are thought to be involved in memory storage. One possible mechanism of this action involves marking a neuron that has been recently active with changes in excitability. This would help to link temporally separated stimuli. Another potential mechanism comes from a computational model that indicates that nonsynaptic plasticity may prime circuits for modification in learning because excitability changes may regulate the threshold for synaptic plasticity.

The storage capacity of synaptic-based memory storage systems is very large, making it an attractive mechanism to study. There are approximately 10⁴ synapses per neuron and 10¹¹ neurons in the human brain. Nonsynaptic plasticity is often overlooked simply because its storage capacity is not as high. Regulating the density of ion channels in the axon and soma of a neuron would change the throughput and affect all of the synapses. Therefore, its storage capacity would be significantly less than that of synaptic plasticity.

While its storage capacity is too low to make it the sole mechanism for storage, nonsynaptic plasticity could contribute to synaptic storage methods. It has been shown that the modulation of ion channels can occur in regions as small as specific dendrites. This specificity makes the storage capacity of nonsynaptic plasticity larger than if it were taken to be whole neuron modulation. Procedural memories are a good fit for this type of storage system because they do not require the high specificity that declarative memories do. Generalization of motor tasks and conditioned stimuli could be an efficient way to store this information.

Learning

Changes in excitability from learning that act as part of the memory trace do so as primers to initiate further changes in the neurons or by a short-term storage mechanism for short-term memory. Nonsynaptic plasticity can emerge during learning as a result of cellular processes, although the timing, persistence, and the relationship between nonsynaptic plasticity and synaptic output are all poorly understood. Studies have shown that nonsynaptic plasticity plays an indirect but important role in the formation of memories. Learning-induced nonsynaptic plasticity is associated with soma depolarization.

Classical conditioning

Experiments have revealed that nonsynaptic changes take place during conditional learning. Woody et al. demonstrated that eyeblink conditioning (EBC), a form of classical conditioning for studying neural structures and mechanisms underlying learning and memory, in a cat is associated with increased excitability and input in the neurons in sensorimotor cortical areas and in the facial nucleus. It was observed that increasing excitability from classical conditioning continued after the response stopped. This suggests that increased excitability may function as a mechanism for memory storage.

In eyeblink conditioning in rabbits, nonsynaptic changes occurred throughout the dorsal hippocampus. This indicates that although excitability changes alone are not enough to explain memory storage processes, nonsynaptic plasticity might be a storage mechanism for phases of memory limited by time. Nonsynaptic changes influence other types of plasticity involved with memory. For example, a nonsynaptic change such as depolarization of the resting membrane potential resulting from conditional learning could cause synaptic plasticity in future learning.

Rule learning and savings

The ability to learn rules is dependent on nonsynaptic plasticity. One study sought to teach rats to discriminate between various odors, and it took several days to teach them to distinguish between a first pair of smells. However, after learning this, the rat was able to learn to distinguish between different odors much faster. Changes in excitability of the pyramidal neurons in these rats were observed for three days after training. These changes faded eventually, suggesting that the neurons were involved in learning the rules, not in storing memory. Daoudal and Debanne attempted to determine if the same learning rules and induction mechanisms defined for synaptic plasticity also applied to nonsynaptic plasticity affecting ion channels. They determined that nonsynaptic and synaptic plasticity share common learning rules and induction pathways, e.g., NMDA receptor dependent long-term potentiation (LTP) and long-term depression (LTD). They also showed that nonsynaptic and synaptic plasticity synergistically form a coherent engram to store memory traces.

Savings is the ability to relearn forgotten information much faster than it was learned originally. Nonsynaptic plasticity is a possible mechanism for this savings effect. During training procedures many neurons experience an increase in intrinsic excitability. This increase in excitability persists even after the memory fades.

Substance dependence

Drugs of abuse typically affect the mesolimbic system, or more specifically, the reward pathway of the nervous system. Amongst the common drugs of abuse, nicotine is one of the strongest agonists at the nicotinic cholinergic synapse. Nicotine, competing with acetylcholine (ACh), acts through the nonsynaptic, preterminal, nicotinic acetylcholine receptor (nAChRs) to initiate a membrane potential change and propagate an intracellular Ca²⁺ signal, thus encouraging the release of neurotransmitters. The specific and characteristic role of calcium current mediated nAChR activity has a different voltage-dependence than other Ca²⁺ permeable ion channels, as well as different temporal and spatial distribution and as a result, the nonsynaptic nAChR activity enhances the induction of synaptic potentiation, promoting the learning of substance dependence.

Applications to disease

After damage

Nonsynaptic plasticity can function to alleviate the effects of brain damage. When one of the vestibular nerves is damaged, disparity in the firing rates of neurons in the vestibular nuclei causes unnecessary vestibular reflexes. The symptoms of this damage fade over time. This is likely due to modifications of intrinsic excitability in the neurons of the vestibular nucleus.

Seizure activity

Nonsynaptic plasticity also plays a key role in seizure activity. Febrile seizures, seizures due to fever early in life, can lead to increased excitability of hippocampal neurons. These neurons become highly sensitized to convulsant agents. It has been shown that seizures early in life can predispose one to more seizures through nonsynaptic mechanisms.

Trauma, including stroke that results in cortical injury, often results in epilepsy. Increased excitability and NMDA conductances result in epileptic activity, suggesting that nonsynaptic plasticity may be the mechanism through which epilepsy is induced after trauma.

Autism

Valproic acid (VPA) is a treatment for epilepsy, migraines, and bipolar disorder that has been linked to many conditions including autism. An animal model of autism exists in which pregnant rats are given VPA. The offspring have traits similar to those of humans with autism. Shortly after birth, these animals exhibit decreased excitability and increased NMDA currents. These effects are corrected at later stages in life. The changes in intrinsic excitability in these animals helped to offset the effects of increased NMDA currents on network activity, a form of homeostatic plasticity. It is believed that this helps mediate the detrimental effects that the increased NMDA currents would have.

Current and future research

Additional research is needed to obtain a broader understanding of nonsynaptic plasticity. Topics that should be further explored as of January 2010 include:

• Local versus global excitability changes in neuronal networks and maintenance of the memory trace
• Specificity of induction of learning-dependent excitability changes
• Manipulation of learning-dependent excitability changes by pharmaceutical products or genetic mutations and their effects on the memory trace
• Similarities between the molecular mechanisms of synaptic and nonsynaptic plasticity
• Comparison of in vivo patterns of nonsynaptic plasticity with in vitro results
• Alterations in gene expression produced by neural activity