Transcription bubble

Termination

Prokaryotic termination

In Escherichia coli, the process of transcription termination via dissociation of the RNA polymerase have been found to depend on 3 possible mechanisms: an interaction between the polymerase and an intrinsic terminator sequence found on the hairpin loops of completed RNA, the presence of the RNA-dependent termination factor Rho, and the ATP-dependent DNA translocase Mfd.

Studies have found that the disruption of the RNAP-DNA transcription complex by termination factor Rho is inhibited for as long as the upstream DNA in the transcription bubble remain unpaired. Thus, the detachment of bacterial RNAP from DNA in a rho-dependent process is preceded by and depends on the re-annealing of DNA within the transcription bubble.

During rho-independent termination, the transcription of a hairpin loop on completed RNA, which serves as the intrinsic termination sequence, contributes to the collapse of the transcription bubble. This is followed by the detachment of RNAP from the template DNA and the re-annealing of DNA strands. This method of termination does not require the presence of the transcription bubble, as E.coli RNAP have been observed in vitro to release the completed RNA transcript while using single-stranded DNA templates.

The third process of termination, involving DNA translocase Mfd, affects primarily transcription bubbles which have stalled in the presence of DNA damage. The presence of Mfd in the transcription bubble forces the downstream movement of RNAP without the addition of nucleoside triphosphates, inducing the re-annealing of the DNA in the transcription bubble and the detachment of both the RNAP and the nascent RNA.

Transcription bubble termination in E. coli is regulated by a variety of transcription factors. One such factor is NusG, a ribosomal protein that enhances the efficiency of Rho-dependent termination by aiding Rho recognition of termination sequences. NusG action is mandatory in situations where RNA release has to be performed in a small window of time.

Eukaryotic termination

Transcription termination by eukaryotic RNA polymerase I (Pol I) requires transcription termination factors similar to rho-dependent termination in prokaryotes. In mice, repeated terminators encoded on DNA are exposed as single-stranded binding sites for protein TTF-I once they are reached by the transcription bubble. The complex produced by the terminator and TTF-I binding then induces the release of the transcript. RNA Polymerase II is terminated through direct binding of the 3′-end cleavage and polyadenylation (CPA) complex to the enzyme, which then releases the transcribed RNA. The recruitment of the CPA complex to the transcription bubble is induced by the transcription of a Poly-A signal on the nascent RNA.

In both cases, RNA cleavage and release occurs before the dissociation of the polymerase from the transcription bubble. Thus, the integrity of the transcription bubble is temporarily preserved after the initiation of termination. Two models have been proposed to explain the process of polymerase dissociation after RNA release for both polymerases. The first is the torpedo model, in which the polymerase continues to synthesize RNA after the release of the nascent RNA. Exonuclease activity then degrades the new RNA strand, destabilizing RNA polymerase and achieving its dissociation from the transcription bubble. The second mechanism, the allosteric model, proposes that transcription of the poly A sequence near the end of nascent RNAs causes gradual dissociation of other transcription factors from the transcription bubble, causing a chain effect that eventually collapses the transcription bubble thorough destabilization.

Regulation

DNA sequence and supercoiling effects

Molecular dynamic simulations have found that the lifetime of the transcription bubble is sequence-dependent, and longer bubble lifetimes are associated with A-T rich core promoter sequences. The weaker A-T base interactions enable transcription bubbles to form due to the less energy needed for A-T pairs to separate. The supercoiling condition of DNA strongly affects how transcription processes regulate. Negative supercoiling that occurs before the transcription start site creates DNA strand separation which leads to transcription initiation. Positive supercoiling in front of RNA polymerase creates a barrier that leads to transcription stalling during elongation. The management of supercoiling stress depends on enzymes including DNA gyrase and topoisomerase. DNA gyrase creates negative supercoils while relaxing positive supercoils to establish the required superhelical tension for effective transcription. Topoisomerase I relax negative supercoils which keeps the DNA structure suitable for transcriptional activities.

Role of transcription factors

The general transcription factors (GTFs) TFIIH function as key elements for transcription initiation in eukaryotic cells. The XPB and XPD helicase subunits of TFIIH enable DNA unwinding through DNA duplex translocation which produces single-stranded regions needed for RNA polymerase II to start transcription. σ factors in bacteria serve as essential components to guide RNA polymerase toward particular promoter sequences which leads to the creation of the transcription bubble and the start of transcription. The protein p53 binds near promoter regions to affect the stability of the transcription bubble while showing different effects on transcription initiation at various target promoters.

A variety of transcription factors also affect the stability of transcription bubble initiation. DksA is crucial for rRNA transcription regulation. It has been found to decrease RNAP complex half-life, thereby inhibiting transcription from rRNA promoters and causing the destabilization of transcription bubble. Similarly, GreA and GreB are homologous factors that have effects similar to DksA, both are also known to reduce RNAP complex half-life. However, it has been discovered that the deletion of GreA and GreB has only minuscule effects on rRNA promoter activity and transcription bubble stability.

Epigenetic modifications and chromatin structure

Epigenetic modifications significantly influence chromatin structure and transcriptional activity. The acetylation of lysine 27 on histone 3 (H3K27ac) creates a less stable nucleosome structure, which leads to the formation of essential transcription bubbles that initiate transcription. The acetylation mark is predominantly found at active promoters together with enhancer regions where it leads to elevated transcriptional activity. The transcriptional impact of promoter region methylation varies based on the specific context and associated proteins present. The SWI/SNF complex functions as a chromatin remodeler to modify nucleosome positions through ATP-dependent mechanisms that remove or reposition nucleosomes to control RNA polymerase access and regulate transcription rates.

Temperature-dependence

The formation and maintenance of the transcription bubble is likely also temperature-dependent: temperature analyses on E.coli DNA suggest that the complex is formed at 37 °C and collapses at lower temperatures. These temperatures may vary depending on species. In conjunction with temperature, the presence of magnesium ions (Mg²⁺) alongside an increase in temperature causes the unwinding of transcription bubbles further downstream up to a base position of +2, which correlates with the start of RNA synthesis. Extended melting at higher temperatures also enhances bubble stability during early transcription stages.

Role in gene expression

In both eukaryotes and prokaryotes, multiple transcription start sites have been observed within the same promoter, and transcription bubble dynamics—such as expansion ("scrunching") and contraction ("unscrunching")—have been shown to play a role in the positioning of these variable transcription start sites to the RNA polymerase active site. As such, the structure of the transcription bubble plays a role in regulating gene expression through mediating the creation of different transcripts.

Scrunching of the transcription bubble is essential to RNAP promoter escape–an obligatory step that releases the RNAP from the promoter to begin elongation of the transcript. Prior to escape, RNAP conducts abortive initiation, where it synthesizes short ~2-9 nt RNA fragments without moving from where it is bound to the promoter. Scrunching of the transcription bubble is essential for this process, to keep the template DNA bases at the RNAP active site without RNAP translocation. The extent of bubble scrunching increases with the size of RNA. Thus, transcription elongation can only occur after sufficient bubble scrunching allows the formation of a large enough RNA product (~10 nt) to trigger promoter escape. The DNA bulges that form the transcription bubble occur at different locations on each strand.

The transcription bubble also generates DNA supercoiling upon RPo formation—a process known to be important to gene regulation. Transcription and bubble movement generates positive supercoiling (overwound helix) ahead of RNAP and negative supercoiling (underwound helix) behind it. The supercoiled structure of the DNA around the transcription bubble has been shown to inhibit elongation when stress is too high. Thus supercoiling due to bubble processes must be managed by topoisomerases.

Evolutionary origins

The first DNA replication origins were hypothesized to be promoters for the 2-double-Ψ-β-barrel (2-DPBB) domains of RNAP. Replication was initiated using 2-DPBB type RNAPs followed by DNA synthesis with reverse transcriptase, providing the earliest known instances of transcription bubbles. 2-DPBB type RNAPs can either be RNA or DNA-template dependent, suggesting that these enzymes, and by extension transcription bubbles, evolved in an RNA world where DNA genomes gradually rose to prominence. Additionally, both DNA and RNA-dependent RNAPs possess a trigger loop and bridge helix, implying that these transcription bubble mechanisms are of ancient origins.

2-DPBB type RNAPs were most likely the primary machinery for transcription and replication of LUCA genome, which suggests that the divergence of bacteria and archaea stems from co-evolution with 2-DPBB type RNAPs, RNAP promoters, and RNAP general transcription factors (GTF). Bacterial promoters were noted to be strong promoters that have contacts with RNAP σA subunits but lack TATA-binding protein (TBP) and Transcription Factor E (TFE). This shows that transcription bubble machinery has been lost during bacterial divergence.

The similar consensus sequences between the Pribnow and TATA boxes found in archaea and eukaryotes respectively caused speculation that both had a common promoter structure in LUCA which diverged at some point in time. Divergence of promoters possibly influenced co-evolution with interacting transcription factors, implying that transcription bubble mechanisms most likely had shared origins stemming from LUCA.

Pharmaceutical significance

Due to the importance of the transcription bubble to the initiation, propagation and termination of transcription, enzymes involved in transcription bubble upkeep are viable targets for drugs that function through gene expression regulation.

Dactinomycin (Actinomycin D)

Dactinomycin is a potent intercalating agent and chemotherapeutic drug that works by inhibiting RNA synthesis. It binds directly to single-stranded DNA in the transcription bubble, the region of DNA where transcription is actively occurring. First isolated in 1940 by chemists Selman A. Waksman and H. Boyd Woodruff from Streptomyces, it has since become widely known for its use in cancer chemotherapy due to its ability to preferentially target and kill tumor cells.

Dactinomycin has wide applicability and is cytotoxic to a wide range of organisms. It is an effective bactericide, capable of inhibiting growth of both Gram positive and Gram negative bacteria, with higher dilutions required to achieve the same antibiotic effects. In eukaryotes, Dactinomycin is preferentially toxic to tumor cells, which makes it an effective chemotherapy drug. Dactinomycin can insert itself between the base pairs of double-stranded or single-stranded DNA, disrupting its normal structure. The drug binds primarily to guanine and cytosine residues found on newly separated single stranded DNA in transcription bubbles. The drug interferes with RNA synthesis machinery upon binding, physically preventing RNA polymerase from moving downstream and effectively inhibiting RNA elongation. This results in the lack of RNA synthesis in the affected cell, resulting in premature cell death.

Aside from its usage in medicine, the ability of Dactinomycin to inhibit RNA production makes it an effective experimental tool for RNA quantification and analysis.

Rifampicin

Rifampicin is a widely used antibiotic that targets bacterial RNA polymerase, inhibiting its ability to synthesize RNA. It is particularly effective against Mycobacterium tuberculosis, the bacterium responsible for tuberculosis, and has been commonly used in combination therapy for tuberculosis and other bacterial infections since 1965. Rifampicin directly binds to the bacterial RNA polymerase subunit β within the transcription bubble immediately after transcription initiation, physically preventing the elongation of the RNA strand past the first few nucleotides. The ongoing evolution of rifampicin-resistant strains of M. tuberculosis continue to present significant challenges to the usage of this drug in tuberculosis treatment. All recorded resistance-conferring mutations are isolated to the sequence of the bacterial RNA polymerase subunit β, the location where Rifampicin physically binds.